Translational_Unit

Part:BBa_K2380042:Design

Designed by: Daniel Kelvin, Elena Nickels, Bea Marie Spiekermann, Lara Steinel, Tim Maier, Patrick Müller, Stefan Zens, Jennifer Zimmermann, Cristina Kurzknabe   Group: iGEM17_TU_Darmstadt   (2017-10-24)


Chitin deacetylase NodB with constitutive Anderson promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the strong constitutive promoter Anderson J23100, because we wanted to express the chitin deacetylase nodB without the need of adding an inducer like IPTG.


Source

see basic parts

References